Goat F(ab)2 anti-Rat IgG (Fc)-HRPO, MinX Bo,Ho,Hu, Polyclonal

Product Overview

• Article Name: Goat F(ab)2 anti-Rat IgG (Fc)-HRPO, MinX Bo,Ho,Hu, Polyclonal
• Biozol Catalog Number: ROC-712-1322
• Supplier Catalog Number: 712-1322
• Alternative Catalog Number: ROC-712-1322
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rat
• Immunogen: Rat IgG F(c) fragment
• Conjugation: HRPO
• Alternative Names: Goat F(ab)2 Anti-Rat IgG F(c) Peroxidase Conjugated Antibody, Goat Fab2 Anti-Rat IgG Fc Antibody HRP Conjugation
• Format: F(ab')2
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Bovine,Equine,Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Rat IgG F(c) Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at

Images

ELISA results of purified F(ab)2 Goat anti-Rat IgG F(c) antibody Peroxidase Conjugated Min x Bv, Hs, & Hu serum proteins tested against purified Rat IgG F(c). Each well was coated in duplicate with 1.0 µg of Rat IgG F(c) (p/n 012-0103). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.