Donkey F(ab)2 anti-Sheep IgG (H+L)-unconj., MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal , Unconjugated

Product Overview

• Article Name: Donkey F(ab)2 anti-Sheep IgG (H+L)-unconj., MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-713-701-168
• Supplier Catalog Number: 713-701-168
• Alternative Catalog Number: ROC-713-701-168
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Sheep IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Donkey F(ab)2 Anti-Sheep IgG Antibody, Donkey Fab2 Anti-Sheep IgG Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Gallus,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 0.95 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Sheep IgG Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated bin

Images

Dot Blot Results of Donkey F(ab)2 Anti-Sheep IgG Antibody Min X Ch GP Ham Hs Hu Ms Rb Rt Serum Proteins. Sheep IgG (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Donkey F(ab)2 Anti-Sheep IgG Antibody at 1µg/mL at RT for 1hr. Secondary Antibody: Anti-Donkey IgG HRP at 1:40,000 for 30 mins at RT. Exposure: 1 sec.