Fab Rabbit IgG (H&L) Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: Fab Rabbit IgG (H&L) Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-811-1602
• Supplier Catalog Number: 811-1602
• Alternative Catalog Number: ROC-811-1602
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Goat Fab Anti-Rabbit IgG Biotin Conjugated Antibody, Goat Fab Fragment Anti-Rabbit IgG Antibody Biotin Conjugation
• Format: Fab
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:90,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Fab Anti-Rabbit IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely l

Images

ELISA Results of Fab Anti-Rabbit IgG (Goat) Antibody Biotin Conjugated. Each well was coated in duplicate with 10 µg of Rabbit IgG (p/n 011-0102). The working dilution is 368,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS (p/n MB-066), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).