Protein C, Activated, Recombinant, Human (PROC, PC, active coagulation factor XIV)

Product Overview

• Article Name: Protein C, Activated, Recombinant, Human (PROC, PC, active coagulation factor XIV)
• Biozol Catalog Number: USB-171986
• Supplier Catalog Number: 171986
• Alternative Catalog Number: USB-171986-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), aa1-461 (end) with C-terminal His tag, expressed in a HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a.43-199, heavy chain a.a.212-461). The signal peptide (1-32) and propeptide (a.a.33-42) is cleaved. MW=~60kD, ~42kD, ~20kD under reducing conditions. Source: Recombinant protein corresponding to aa1-461 of PROC at C-terminus, fused to His-tag expressed in a HEK293 cell expression system. Molecular Weight: ~60kD, ~42kD, ~20kD under reducing conditions. Specific Activity: 95pmol/min/ug Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

Product Properties

• Molecular Weight: 604624
• NCBI: 000312
• UniProt: P04070
• Purity: ~90%
• Form: Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 138mM NaCl, 2.7mM KCl, 250mM Imidazole, 0.05% Brij-35.