Recombinant protein corresponding to Ser704-Ile822 from human AAK1, expressed in E. coli.
AP2-associated protein kinase 1 (AAK1) is a 100kD intracellular kinase that binds and phosphorylates NUMB and the m2 subunit of AP2. NUMB and AP2 are adaptor proteins which are involved in the assembly and sorting of clathrin coated pits during receptor-mediated endocytosis. AAK1 contains one catalytic domain aa46-315 and a C-terminal clathrin-binding region. Alternate splicing of human AAK1 generates a 145kD long isoform that contains an additional clathrin-binding domain. Within aa704-822, human AAK1 shares 95% aa sequence identity with mouse and rat AAK1. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using IMR-32 human neuroblastoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
