MCP2 (Macrophage/Monocyte Chemotactic Protein 2, Monocyte Chemotactic Protein 2, MCP-2, C-C Motif Chemokine 8, Chemokine (C-C Motif) Ligand 8, CCL8, HC14, Monocyte Chemoattractant Protein 2, Small Inducible Cytokine A8, SCYA8, SCY
Biozol Catalog Number:
USB-M2750-08A
Supplier Catalog Number:
M2750-08A
Alternative Catalog Number:
USB-M2750-08A-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
Recombinant protein corresponding to human MCP-2, expressed in E. coli.
MCP-2 acts as a chemoattractant for monocytes, lymphocytes, eosinophils and basophils, due to its ability to bind to the common MCP receptor, chemokine (C-C motif) receptor 2 (CCR2), and also CCR1 and CCR3. Proteolytic cleavage of MCP-2 into the NH2-terminally truncated form, MCP-2 (6-76), results in the loss of chemotactic activity, forming a functional C-C chemokine inhibitor, which may be important during inflammatory responses. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml, in conjunction with M2750-07, allows the detection of at least 0.2-0.4ng/well of recombinant hMCP-2 Western Blot: 0.1-0.2ug/ml with compatible secondary reagents the detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
