C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, TPO
TN1 was prepared by immunizing BALB/c mice by intraperitoneal injecction of recombinant human TPO. The Fab fragment was produced via incubation with papain which cleaves at the hinge region of the IgG to remove the Fc region. The product is then subsequently incubated under reducing conditions and the free Cys residue blocked with iodoacetamide.
