Human Leukocyte antigen, HLA-A2, HLA-A28, HLA A2, HLA A28
A 57-year-old female presented with post-transfusion purpura 5 days following a blood transfusion. Serological studies with the sera of this patient identified the presence of anti-HLA-A2. A 50-ml EDTA anti-coagulated blood sample was obtained from the patient in the acute phase of her PTP, and mononuclear cells were isolated from the blood sample by layering onto a Ficoll gradient. An oligo dT primer was used to direct first strand cDNA synthesis and IgG encoded VH was amplified and an scFv library was constructed. Phage particles expressing anti-HLA A2/A28 scFvs were selected by panning.
Clonality:
Monoclonal
Clone Designation:
[3PF12]
Isotype:
IgG
Buffer:
PBS only.
Source:
Human
Target:
HLA-A2/A28
Antibody Type:
Recombinant Antibody
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