Anti-C5a [Fab 103], Rabbit IgG, kappa, Monoclonal
Catalog Number:
ABA-AB00990-23.0-BT
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| Article Name: | Anti-C5a [Fab 103], Rabbit IgG, kappa, Monoclonal |
| Biozol Catalog Number: | ABA-AB00990-23.0-BT |
| Supplier Catalog Number: | Ab00990-23.0-BT |
| Alternative Catalog Number: | ABA-AB00990-23.0-BT |
| Manufacturer: | Absolute Antibody |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | Blocking, ELISA |
| Species Reactivity: | Human |
| Alternative Names: | Complement Component 5a |
| A panel of mAbs against the activated complement component C5a was obtained from a filamentous phage M13-Fab display library generated from mice immunized with human rC5a. Fabs isolated from the library after iterative selection against rC5a bound to both rC5a and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a but not on the native complement component C5, the library was rescreened in a competitive manner. The phage Fab library was first incubated with immobilized C5 to deplete C5 reactive Fabs. The C5 nonadherent phage were then incubated with immobilized rC5a in the presence of soluble C5. Bound phage were eluted and subjected to two additional cycles of subtraction with immobilized C5 and selection with immobilized rC5a in the presence of soluble C5. After three cycles of this competitive biopanning, Fabs reactive with rC5a were isolated. |
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