The original antibody was generated by immunizing BDF1 mice intraperitoneally with a MPV55-KLH emulsified in complete Freunds adjuvant. MPV55-KLH is a mouse vimentin phosphopeptide MPV55 (Ser-Leu-Tyr-Ser-Ser-phosphoSef15-ProGly-Gly-Ala-Tyr-Cys) which was coupled to keyhole limpet hemocyanin (KLH) with m-maleimido-benzoyl-N-hydroxysuccinimide ester.