The original antibody was generated by immunizing XenoMouse mice with SK-MEL-28 cells admixed 1:1 v/v with Complete Freunds Adjuvant (CFA), followed by four injections with 5 µg soluble MUC18-human IgG2 Fc fusion protein admixed 1:1 v/v with Incomplete Freunds Adjuvant (IFA) and then a final boost of 10 µg soluble MUC18-human IgG2 Fc fusion protein without adjuvant.