The original antibody was generated by cloning the variable light and heavy chain genes of the anti-DOM hybridoma were assembled into the scFv gene using a linker sequence (Gly4Ser)3 by over-lapping PCR. PCR amplified products were purified, treated with SfiI and NotI and cloned into phagemid expression pDisplay-2TM . The plasmid was used to transform E.coli HB2151 host cells and the soluble scFv was generated.
Clonality:
Monoclonal
Clone Designation:
[AbDON01]
Isotype:
IgG1
Buffer:
PBS with 0.02% Proclin 300.
Source:
Mouse
Target:
Deoxynivalenol
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