VEGFR1 Rabbit pAb, Unconjugated

Product Overview

• Article Name: VEGFR1 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A0058
• Supplier Catalog Number: A0058
• Alternative Catalog Number: ABB-A0058-100UL, ABB-A0058-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: FLT, FLT-1, VEGFR1, VEGFR-1

Product Description

This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

Product Properties

• Molecular Weight: 151kDa
• NCBI: 2321
• UniProt: P17948
• Purity: Affinity purification
• Target: FLT1

Application Notes

• Application Dilute: WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Growth factors,Endocrine Metabolism,Cardiovascular,Angiogenesis,Hypoxia.

Images

Western blot analysis of various lysates using VEGFR1 Rabbit pAb (A0058) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Immunofluorescence analysis of NIH/3T3 cells using VEGFR1 Rabbit pAb (A0058) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using VEGFR1 Rabbit pAb (A0058) at dilution of 1:100. Blue: DAPI for nuclear staining.