SSNA1 Rabbit pAb, Unconjugated

Product Overview

• Article Name: SSNA1 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A13377
• Supplier Catalog Number: A13377
• Alternative Catalog Number: ABB-A13377-100UL,ABB-A13377-20UL,ABB-A13377-1000UL,ABB-A13377-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: N14, NA14, NA-14, SSNA1

Product Description

Enables identical protein binding activity. Predicted to act upstream of or within ciliary receptor clustering involved in smoothened signaling pathway and intraciliary transport. Located in centrosome.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 14kDa
• NCBI: 8636
• UniProt: O43805
• Purity: Affinity purification
• Sequence: MTQQGAALQNYNNELVKCIEELCQKREELCRQIQEEEDEKQRLQNEVRQLTEKLARVNENLARKIASRNEFDRTIAETEAAYLKILESSQTLLSVLKREAGNLTKATAPDQKSSGGRDS
• Target: SSNA1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cell Biology Developmental Biology,Cell Cycle,Centrosome,Immunology Inflammation.

Images

Western blot analysis of various lysates using SSNA1 Rabbit pAb (A13377) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded Rat lung using SSNA1 Rabbit pAb (A13377) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Western blot analysis of lysates from mouse spleen, using SSNA1 Rabbit pAb (A13377) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using SSNA1 Rabbit pAb (A13377) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using SSNA1 Rabbit pAb (A13377) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.