[KD Validated] TRIM21/SS-A Rabbit pAb, Unconjugated

Article Name:	[KD Validated] TRIM21/SS-A Rabbit pAb, Unconjugated
Biozol Catalog Number:	ABB-A1957
Supplier Catalog Number:	A1957
Alternative Catalog Number:	ABB-A1957-100UL,ABB-A1957-20UL,ABB-A1957-500UL,ABB-A1957-1000UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	SSA, RO52, SSA1, RNF81, Ro/SSA, TRIM21/SS-A

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Clonality:	Polyclonal
Molecular Weight:	54kDa
NCBI:	6737
UniProt:	P19474
Purity:	Affinity purification
Sequence:	GTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGA
Target:	TRIM21
Antibody Type:	Primary Antibody

Application Dilute:	WB,1:500 - 1:5000\|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells\|IF/ICC,1:50 - 1:200\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Immunology Inflammation.

	Western blot analysis of lysates from wild type (WT) and TRIM21/SS-A knockout (KO) 293T cells using TRIM21/SS-A Rabbit pAb (A1957) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Western blot analysis of lysates from HeLa cells using TRIM21/SS-A Rabbit pAb (A1957) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Western blot analysis of lysates from Rat lung usingTRIM21/SS-A Rabbit pAb (A1957) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:180s.
	Immunofluorescence analysis of NIH/3T3 cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of THP-1 cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunoprecipitation of TRIM21/SS-A in 200 µg extracts from Hep G2 cells using 0.5 µg TRIM21/SS-A Rabbit pAb (A1957). Western blot analysis was performed using TRIM21/SS-A Rabbit pAb (A1957) at 1:1500 dilution.
	Immunofluorescence analysis of U2OS cells using TRIM21/SS-A Rabbit pAb (A1957) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunoprecipitation of TRIM21/SS-A in 200 µg extracts from Hep G2 cells using 0.5 µg TRIM21/SS-A Rabbit pAb (A1957). Western blot analysis was performed using TRIM21/SS-A Rabbit pAb (A1957) at 1:1500 dilution.