[KO Validated] HuR/ELAVL1 Rabbit mAb, Unconjugated

Product Overview

• Article Name: [KO Validated] HuR/ELAVL1 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A19622
• Supplier Catalog Number: A19622
• Alternative Catalog Number: ABB-A19622-100UL,ABB-A19622-20UL,ABB-A19622-500UL,ABB-A19622-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, IP, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HUR, Hua, MelG, ELAV1, L1

Product Description

The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3 untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC0101]
• Molecular Weight: 36kDa
• NCBI: 1994
• UniProt: Q15717
• Purity: Affinity purification
• Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS
• Target: ELAVL1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:6000|IHC-P,1:200 - 1:2000|IF/ICC,1:100 - 1:1000|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Endocrine Metabolism,AMPK Signaling Pathway.

Images

Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622,dilution 1:100)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622,dilution 1:100)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunoprecipitation analysis of 300 µg extracts of 293T cells using 3 µg HuR/ELAVL1 antibody (A19622). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody (A19622) at a dilution of 1:1000.