SARS-CoV-2 Spike RBD Rabbit pAb

Product Overview

• Article Name: SARS-CoV-2 Spike RBD Rabbit pAb
• Biozol Catalog Number: ABB-A20135
• Supplier Catalog Number: A20135
• Alternative Catalog Number: ABB-A20135-20UL,ABB-A20135-100UL,ABB-A20135-500UL,ABB-A20135-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IP, WB
• Species Reactivity: Virus
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Alternative Names: sars-cov-2, spike glycoprotein, SARS-CoV-2 Spike RBD

Product Description

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 141kDa
• NCBI: 43740568
• UniProt: P0DTC2
• Purity: Affinity purification
• Sequence: ITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTG
• Target: Spike RBD
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,1:50000-1:200000
• Application Notes: Cross-Reactivity: SARS-CoV-2

Images

Immunofluorescence analysis of 293T-RBD and 293T cells using SARS-CoV-2 Spike RBD Rabbit pAb (A20135) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300 µg extracts of 293T cells using 3 µg SARS-CoV-2 Spike RBD antibody (A20135). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike RBD antibody (A20135) at a dilution of 1:3000.

Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike RBD Protein,using SARS-CoV-2 Spike RBD Rabbit pAb (A20135) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Immobilized Recombinant SARS-COV-2 Spike RBD Protein (RP01278LQ) at 1µg/mL (100µL/well) can bind SARS-CoV-2 Spike RBD Rabbit pAb (A20135) with a linear range of 0.78-50ng/mL.