SARS-CoV-2 Nucleoprotein Rabbit mAb

Product Overview

• Article Name: SARS-CoV-2 Nucleoprotein Rabbit mAb
• Biozol Catalog Number: ABB-A21042
• Supplier Catalog Number: A21042
• Alternative Catalog Number: ABB-A21042-20UL, ABB-A21042-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Virus
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Alternative Names: N, Nucleoprotein, NC, SARS-CoV-2 Nucleoprotein, sars-cov-2

Product Description

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.

Product Properties

• Molecular Weight: 46kDa
• NCBI: 43740575
• UniProt: P0DTC9
• Purity: Affinity purification
• Target: Nucleoprotein

Application Notes

• Application Dilute: WB,1:2000 - 1:10000|IF/ICC,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: SARS-CoV-2, ResearchArea: Microbiology ,Organism ,Virus ,RNA Virus, ssRNA positive strand virus, SARS Coronavirus.

Images

Western blot analysis of lysates from 293T cells, using SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.2s.

Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042) at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.