DiMethyl-Histone H3-K79 Rabbit pAb, Unconjugated

Product Overview

• Article Name: DiMethyl-Histone H3-K79 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A2368
• Supplier Catalog Number: A2368
• Alternative Catalog Number: ABB-A2368-100UL,ABB-A2368-20UL,ABB-A2368-500UL,ABB-A2368-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, IF, IHC-P, IP, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3, DiMethyl-Histone H3-K79

Product Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 15 kDa
• NCBI: 8290
• UniProt: Q16695
• Purity: Affinity purification
• Sequence: DFKTD
• Target: H3-4
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|IHC-P,1:50 - 1:100|IF/ICC,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.|ChIP,5
• Application Notes: Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted), ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Signal Transduction,MAPK-Erk Signaling Pathway.

Images

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (A2368).

Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using DiMethyl-Histone H3-K79 Rabbit pAb (A2368) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western blot analysis of lysates from HeLa cells, using DiMethyl-Histone H3-K79 Rabbit pAb (A2368) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded Rat testis using DiMethyl-Histone H3-K79 Rabbit pAb (A2368) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using DiMethyl-Histone H3-K79 Rabbit pAb (A2368) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 Rabbit pAb (A2368). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300 µg extracts of HeLa cells using 3 µg DiMethyl-Histone H3-K79 antibody (A2368). Western blot was performed from the immunoprecipitate using DiMethyl-Histone H3-K79 antibody (A2368) at a dilution of 1:1000.

Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using DiMethyl-Histone H3-K79 antibody (A2368) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and DiMethyl-Histone H3-K79 Rabbit pAb (A2368). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K79 in selected genomic region and representative gene loci (GAPDH), as shown in figure.