[KO Validated] PCIF1 Rabbit mAb, Unconjugated

Product Overview

• Article Name: [KO Validated] PCIF1 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A24070
• Supplier Catalog Number: A24070
• Alternative Catalog Number: ABB-A24070-100UL,ABB-A24070-20UL,ABB-A24070-500UL,ABB-A24070-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC-P, IP, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CAPAM, hCAPAM, hPCIF1, C20orf67, PPP1R121, F1

Product Description

Enables RNA polymerase II C-terminal domain phosphoserine binding activity, S-adenosyl-L-methionine binding activity, and mRNA (2-O-methyladenosine-N6-)-methyltransferase activity. Involved in mRNA methylation, negative regulation of translation, and positive regulation of translation. Located in intercellular bridge, microtubule cytoskeleton, and nucleoplasm.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC64786]
• Molecular Weight: 81kDa
• NCBI: 63935
• UniProt: Q9H4Z3
• Purity: Affinity purification
• Sequence: MANENHGSPREEASLLSHSPGTSNQSQPCSPKPIRLVQDLPEELVHAGWEKCWSRRENRPYYFNRFTNQSLWEMPVLGQHDVISDPLGLNATPLPQDSSLVETPPAENKPRKRQLSEEQPSGNGVKKPKIEIPVTPTGQSVPSSPSIPGTPTLKMWGTSPEDKQQAALLRPTEVYWDLDIQTNAVIKHRGPSEVLPPHPE
• Target: PCIF1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|IHC-P,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling.

Images

Western blot analysis of lysates from wild type (WT) and PCIF1 knockout (KO) 293T cells (RM01804) using [KO Validated] PCIF1 Rabbit mAb (A24070) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] PCIF1 Rabbit mAb (A24070) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Western blot analysis of various lysates using [KO Validated] PCIF1 Rabbit mAb (A24070) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:45s.

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] PCIF1 Rabbit mAb (A24070) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] PCIF1 Rabbit mAb (A24070) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] PCIF1 Rabbit mAb (A24070) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] PCIF1 Rabbit mAb (A24070) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KO Validated] PCIF1 Rabbit mAb (A24070) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunoprecipitation of PCIF1 from 200 µg extracts of NIH/3T3 cells was performed using 0.5 µg of [KO Validated] PCIF1 Rabbit mAb (A24070). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KO Validated] PCIF1 Rabbit mAb (A24070) at a dilution of 1:1000.