Megalin/LRP2 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Megalin/LRP2 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A24690
• Supplier Catalog Number: A24690
• Alternative Catalog Number: ABB-A24690-100UL, ABB-A24690-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, FC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: DBS, GP330, LRP-2, Megalin/LRP2

Product Description

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling, extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).

Product Properties

• Molecular Weight: 522kDa
• NCBI: 4036
• UniProt: P98164
• Purity: Affinity purification
• Target: LRP2

Application Notes

• Application Dilute: WB,1:500 - 1:1000|IF-P,1:50 - 1:200|IHC-P,1:50 - 1:200|FC (intra),1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Cell Biology Developmental Biology,Endocrine Metabolism,Lipid Metabolism,Endocrine and metabolic diseases,Obesity,Stem Cells,Cardiovascular,Lipids,Fatty Acids.

Images

Western blot analysis of lysates from Mouse kidney usingMegalin/LRP2 Rabbit mAb (A24690) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:20s.

Immunohistochemistry analysis of paraffin-embedded Human kidney using Megalin/LRP2 Rabbit mAb (A24690) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver using Megalin/LRP2 Rabbit mAb (A24690) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Megalin/LRP2 Rabbit mAb (A24690) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver using Megalin/LRP2 Rabbit mAb (A24690) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Confocal imaging of mouse kidney using Megalin/LRP2 Rabbit mAb (A24690,at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.

Confocal imaging of rat kidney using Megalin/LRP2 Rabbit mAb (A24690,at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.

Flow cytometry: 1X10 6 Reh cells (Low Expression,left) and Caco-2 (right) cells were intracellularly-stained with Megalin/LRP2 Rabbit mAb (A24690,2 µg/mL,orange line) or ABflo 647 Rabbit IgG isotype control (A22070,5 µl/Test,blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10 6 Caco-2 cells were intracellularly-stained with ABflo 647 Rabbit IgG isotype control (A22070,5 µl/Test,left) or Megalin/LRP2 Rabbit mAb (A24690,2 µg/mL,right).