[KD Validated] TGF beta 1 Rabbit mAb, Unconjugated

Article Name:	[KD Validated] TGF beta 1 Rabbit mAb, Unconjugated
Biozol Catalog Number:	ABB-A25313
Supplier Catalog Number:	A25313
Alternative Catalog Number:	ABB-A25313-1000UL,ABB-A25313-100UL,ABB-A25313-20UL,ABB-A25313-500UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	CED, LAP, DPD1, TGFB, IBDIMDE, TGFbeta, TGF-beta1, TGF beta 1

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Molecular Weight:	44kDa
NCBI:	7040
UniProt:	P01137
Purity:	Affinity purification
Target:	TGFB1

Application Dilute:	WB,1:500 - 1:1000\|IF/ICC,1:200 - 1:400\|IHC-P,1:1000 - 1:5000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Growth factors,TGF-b-Smad Signaling Pathway,Wnt -Catenin Signaling Pathway,Endocrine Metabolism,Stem Cells,Cardiovascular,Angiogenesis,Hypoxia.

	Western blot analysis of various lysates using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:90s.
	Western blot analysis of lysates from wild type (WT) and TGF beta 1 knockdown (KD) 293T cells using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of lysates from NIH/3T3 cells using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
	Immunohistochemistry analysis of paraffin-embedded Human bone marrow tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat bone marrow tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of paraffin-embedded Mouse spleen tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Rat spleen tissue using [KD Validated] TGF beta 1 Rabbit mAb (A25313, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.