Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
Gmcsf, Gm-csf
Enables cytokine activity. Involved in several processes, including cellular response to lipopolysaccharide, macrophage activation, and response to silicon dioxide. Located in extracellular space. Used to study Parkinsonism and myocardial infarction. Biomarker of abdominal aortic aneurysm, interstitial cystitis, and lung disease (multiple). Human ortholog(s) of this gene implicated in acute myeloid leukemia, juvenile myelomonocytic leukemia, neutropenia, pulmonary alveolar proteinosis, and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).
WB,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Rat. ResearchArea: cytokine activity , granulocyte macrophage colony stimulating factor receptor binding , growth factor activity , cell response to granulocyte macrophage colony stimulating factor stimulation , cell response to lipopolysaccharide , dendritic cell differentiation , embryonic placenta development , epithelial cell proliferation , epithelial fluid transport , granulocyte-macrophage colony stimulating factor signaling pathway , histamine secretion , immune response , macrophage activation , macrophage differentiation , monocyte differentiation , myeloid cell differentiation , myeloid dendritic cell differentiation , the negative regulation of exogenous apoptosis signaling pathway in the absence of ligand , negative transcriptional regulation, dna template , neutrophil differentiation , positive regulation of cell proliferation , positive regulation of gene expression. Shipping: Ice Bag
Western blot analysis of recombinant Rat GM-CSF Protein using GM-CSF Rabbit mAb (A25503) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 5 ng/1 ng/0.1 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
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