AGER Rabbit PolymAb, Unconjugated

Product Overview

• Article Name: AGER Rabbit PolymAb, Unconjugated
• Biozol Catalog Number: ABB-A26429PM
• Supplier Catalog Number: A26429PM
• Alternative Catalog Number: ABB-A26429PM-100UL, ABB-A26429PM-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: RAGE, sRAGE, SCARJ1

Product Description

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimers disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847).

Product Properties

• Molecular Weight: 43kDa
• NCBI: 177
• UniProt: Q15109
• Purity: Affinity purification
• Target: AGER

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|IF-P,1:600 - 1:2400|IHC-P,1:10000 - 1:40000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Endocrine Metabolism,Endocrine and metabolic diseases,Diabetes,Immunology Inflammation,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Cardiovascular.

Images

Western blot analysis of various lysates using AGER Rabbit PolymAb (A26429PM)at 1:1000 dilutionincubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): K-562
Exposure time: 45s.

Immunohistochemistry analysis of paraffin-embedded Human lung tissue using AGER Rabbit PolymAb (A26429PM) at a dilution of 1:20000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using AGER Rabbit PolymAb (A26429PM) at a dilution of 1:20000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using AGER Rabbit PolymAb (A26429PM) at a dilution of 1:20000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Confocal imaging of paraffin-embedded Human lung tissue using AGER Rabbit PolymAb (A26429PM, dilution 1:1200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Rat lung tissue using AGER Rabbit PolymAb (A26429PM, dilution 1:1200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Mouse lung tissue using AGER Rabbit PolymAb (A26429PM, dilution 1:1200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.