Cofilin Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: Cofilin Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A2658
• Supplier Catalog Number: A2658
• Alternative Catalog Number: ABB-A2658-20UL,ABB-A2658-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CFL, cofilin, HEL-S-15, Cofilin

Product Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 19kDa
• NCBI: 1072
• UniProt: P23528
• Purity: Affinity purification
• Target: CFL1

Application Notes

• Application Dilute: WB,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Protein phosphorylation,Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Cell Adhesion,Cytoskeleton,Microfilaments,Microtubules,Actins,TGF-b-Smad Signaling Pathway,Immunology Inflammation,Neuroscience, Cell Type Marker,Cardiovascular,Heart,Contractility,Neuron marker. Shipping: Ice Bag

Images

Western blot analysis of various lysates using Cofilin Rabbit mAb (A2658) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.