AFG3L2 Rabbit PolymAb, Unconjugated

Product Overview

• Article Name: AFG3L2 Rabbit PolymAb, Unconjugated
• Biozol Catalog Number: ABB-A26628PM
• Supplier Catalog Number: A26628PM
• Alternative Catalog Number: ABB-A26628PM-1000UL,ABB-A26628PM-20UL,ABB-A26628PM-100UL,ABB-A26628PM-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, IP, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: OPA12, SCA28, SPAX5

Product Description

This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

Product Properties

• Molecular Weight: 89kDa
• NCBI: 10939
• UniProt: Q9Y4W6
• Purity: Affinity purification
• Sequence: DSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPG
• Target: AFG3L2

Application Notes

• Application Dilute: WB,1:15000 - 1:60000|IP,0.5µg-4µg antibody for 400µg-800µg extracts of whole cells|IF-P,1:3200 - 1:12800|IHC-P,1:4000 - 1:40000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requireme
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Signal Transduction,Cell Biology Developmental Biology,Ubiquitin,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Neuroscience,Neurodegenerative Diseases,Neurodegenerative Diseases Markers,Other Neurological disorders.

Images

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using AFG3L2 Rabbit PolymAb (A26628PM) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Western blot analysis of various lysates using AFG3L2 Rabbit PolymAb (A26628PM) at 1:15000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using AFG3L2 Rabbit PolymAb (A26628PM) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using AFG3L2 Rabbit PolymAb (A26628PM) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using AFG3L2 Rabbit PolymAb (A26628PM) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using AFG3L2 Rabbit PolymAb (A26628PM) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using AFG3L2 Rabbit PolymAb (A26628PM) at a dilution of 1:16000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Confocal imaging of paraffin-embedded Human kidney tissue using AFG3L2 Rabbit PolymAb (A26628PM, dilution 1:3200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

Immunoprecipitation of AFG3L2 from 600 µg extracts of Mouse liver tissue was performed using 1 µg of AFG3L2 Rabbit PolymAb (A26628PM). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using AFG3L2 Rabbit PolymAb (A26628PM) at a dilution of 1:10000.