SMARCAD1 Rabbit mAb, Unconjugated

Article Name:	SMARCAD1 Rabbit mAb, Unconjugated
Biozol Catalog Number:	ABB-A26920
Supplier Catalog Number:	A26920
Alternative Catalog Number:	ABB-A26920-20UL, ABB-A26920-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	HRZ, TYS, ETL1, HEL1, ADERM, BASNS

This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight:	68 kDa/117 kDa
NCBI:	56916
UniProt:	Q9H4L7
Purity:	Affinity purification
Target:	SMARCAD1

Application Dilute:	WB,1:25000 - 1:50000\|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells\|IF/ICC,1:200 - 1:800\|IHC-P,1:200 - 1:800\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Chromatin Remodeling.

	Western blot analysis of lysates from Jurkat cells using SMARCAD1 Rabbit mAb (A26920) at 1:50000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using SMARCAD1 Rabbit mAb (A26920) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon tissue using SMARCAD1 Rabbit mAb (A26920) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using SMARCAD1 Rabbit mAb (A26920) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using SMARCAD1 Rabbit mAb (A26920) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using SMARCAD1 Rabbit mAb (A26920) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of Jurkat cells using SMARCAD1 Rabbit mAb (A26920, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of 293T cells using SMARCAD1 Rabbit mAb (A26920, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Immunoprecipitation of SMARCAD1 from 300 µg extracts of Jurkat cells was performed using 2 µg of SMARCAD1 Rabbit mAb (A26920). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using SMARCAD1 Rabbit mAb (A26920) at a dilution of 1:50000.