Caspase-1 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: Caspase-1 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A27901
• Supplier Catalog Number: A27901
• Alternative Catalog Number: ABB-A27901-500UL,ABB-A27901-1000UL,ABB-A27901-100UL,ABB-A27901-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ICE, P45, IL1BC

Product Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 45kDa
• NCBI: 834
• UniProt: P29466
• Purity: Affinity purification
• Sequence: NPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKD
• Target: CASP1

Application Notes

• Application Dilute: WB,1:2500 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human. ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Caspases,Endocrine Metabolism,Immunology Inflammation,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag

Images

Western blot analysis of lysates from THP-1 cells using Caspase-1 Rabbit mAb (A27901) at 1:5000 dilutionincubated overnight at 4°C. THP-1 cells were treated with LPS (1 µg/ml) at 37°C for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.