COL2A1 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A28341
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| Article Name: | COL2A1 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A28341 |
| Supplier Catalog Number: | A28341 |
| Alternative Catalog Number: | ABB-A28341-100UL,ABB-A28341-20UL,ABB-A28341-500UL,ABB-A28341-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Mouse |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | Dmm, Lpk, Col2, Del1, Col2a, Col2a-1, M100413, Rgsc413, Rgsc856 |
| This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. |
| Application Dilute: | WB,1:1000 - 1:3000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (1:10000),a sequential dilution method is strongly recommended to ensure |
| Application Notes: | Cross-Reactivity: Mouse,Rat. ResearchArea: Metabolism. Shipping: Ice Bag |
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Western blot analysis of various lysates using COL2A1 Rabbit pAb (A28341) at 1:1000 dilutionincubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): Mouse liver. Exposure time: 90 s. |
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