Cleaved-Caspase 3 p12 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: Cleaved-Caspase 3 p12 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A28349
• Supplier Catalog Number: A28349
• Alternative Catalog Number: ABB-A28349-1000UL,ABB-A28349-20UL,ABB-A28349-500UL,ABB-A28349-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CPP32, SCA-1, CPP32B

Product Description

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 32 kDa
• NCBI: 836
• UniProt: P42574
• Purity: Affinity purification
• Target: CASP3

Application Notes

• Application Dilute: WB,1:2000 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (1:10000),a sequential dilution method is strongly recommended to ensur
• Application Notes: Cross-Reactivity: Human. ResearchArea: Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Caspases,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease. Shipping: Ice Bag

Images

Western blot analysis of lysates from HeLa cells using Cleaved-Caspase 3 p12 Rabbit mAb (A28349) at 1:5000 dilutionincubated overnight at 4°C. HeLa cells were treated with Staurosporine (3 µM) for 3 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60 s.