PLIN5 Rabbit pAb, Unconjugated

Product Overview

• Article Name: PLIN5 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A28371
• Supplier Catalog Number: A28371
• Alternative Catalog Number: ABB-A28371-20UL,ABB-A28371-500UL,ABB-A28371-100UL,ABB-A28371-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Mouse
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: MLDP, Lsdp5, PAT-1, 2310076L09Rik

Product Description

Enables identical protein binding activity and lipase binding activity. Involved in several processes, including negative regulation of peroxisome proliferator activated receptor signaling pathway, positive regulation of triglyceride storage, and regulation of lipid metabolic process. Located in cytosol. Colocalizes with lipid droplet. Is expressed in brown fat. Orthologous to human PLIN5 (perilipin 5).

Product Properties

• Molecular Weight: 50 kDa/49 kDa
• NCBI: 66968
• UniProt: Q8BVZ1
• Purity: Affinity purification
• Target: Plin5

Application Notes

• Application Dilute: WB,1:1000 - 1:3000|IF-P,1:100 - 1:300|IHC-P,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (1:10000),a sequential dilution
• Application Notes: Cross-Reactivity: Mouse,Rat, ResearchArea: Endocrine Metabolism,Lipid Metabolism,Cardiovascular,Lipids.

Images

Western blot analysis of various lysates using PLIN5 Rabbit pAb (A28371) at 1:3000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90 s.

Immunohistochemistry analysis of paraffin-embedded Mouse brown adipose tissue using PLIN5 Rabbit pAb (A28371) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using PLIN5 Rabbit pAb (A28371) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using PLIN5 Rabbit pAb (A28371) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse skeletal muscle tissue using PLIN5 Rabbit pAb (A28371) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brown adipose tissue using PLIN5 Rabbit pAb (A28371) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat skeletal muscle tissue using PLIN5 Rabbit pAb (A28371) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunofluorescence analysis of Mouse heart tissue using PLIN5 Rabbit pAb (A28371) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

Immunofluorescence analysis of Rat heart tissue using PLIN5 Rabbit pAb (A28371) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.