Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
ABflo 647
Alternative Names:
If2f, IFNG2, IFN gamma
Interferon-gamma (IFN-gamma) is a potent pleiotropic cytokine primarily secreted by activated NK cells and T lymphocytes. Initially characterized based on its antiviral activity, IFN-gamma also exhibits antiproliferative, immunoregulatory, and pro-inflammatory properties. It can upregulate the expression of both MHC class I and class II molecules on antigen-presenting cells. The DB-1 antibody reacts with rat and mouse interferon-gamma (IFN-gamma) and is capable of neutralizing the biological activity of both native and recombinant IFN-gamma. The DB-1 antibody has been well-characterized in applications including ELISPOT, ELISA, intracellular staining, Western blot, immunohistochemistry (IHC), and neutralization assays (both in vitro and in vivo).
FC (intra),0.5 µg per million cells in 100 µl volume
Application Notes:
Cross-Reactivity: Rat. ResearchArea: Infections and Immunity, Autoimmune and Inflammatory Diseases, Cancer Immunology / Tumor Immunology, Immune Regulation and Cell Function. Shipping: Ice Bag
Flow cytometry:1X10 6 Rat splenocytes (untreated,left) and Rat splenocytes (treated with 20ng/ml PMA, 1µg/ml Ionomycin and 5µg/ml Brefeldin A for 6 hours,right) were intracellularly-stained with ABflo 488 Rabbit anti-Rat CD4 mAb (A28295,0.5 µg) and ABflo 647 Rabbit IgG isotype control (A22070,5 µl/Test,left) or ABflo 647 Rabbit anti-Rat IFN-gamma mAb (A28382,0.5 µg,right).
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