IRS1 Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: IRS1 Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A28406
• Supplier Catalog Number: A28406
• Alternative Catalog Number: ABB-A28406-100UL,ABB-A28406-20UL,ABB-A28406-1000UL,ABB-A28406-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Mouse
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: G972R, IRS-1

Product Description

Enables phosphatidylinositol 3-kinase activator activity, phosphatidylinositol 3-kinase binding activity, and transmembrane receptor protein tyrosine kinase adaptor activity. Involved in cellular response to fatty acid, insulin receptor signaling pathway, and insulin-like growth factor receptor signaling pathway. Acts upstream of or within several processes, including cellular response to insulin stimulus, positive regulation of epithelial cell migration, and positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction. Located in several cellular components, including ciliary basal body, nucleus, and plasma membrane. Is expressed in several structures, including alimentary system, brain, genitourinary system, hemolymphoid system gland, and liver and biliary system. Used to study type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in Alzheimers disease, coronary artery disease, type 2 diabetes mellitus, and uterine fibroid. Orthologous to human IRS1 (insulin receptor substrate 1).

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 131 kDa
• NCBI: 16367
• UniProt: P35569
• Purity: Affinity purification
• Target: Irs1

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (1:10000),a sequential dilution method is strongly recommended to ensure
• Application Notes: Cross-Reactivity: Mouse. ResearchArea: Metabolism. Shipping: Ice Bag

Images

Western blot analysis of various lysates using IRS1 Rabbit pAb (A28406) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45 s.