IRF1 Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: IRF1 Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A28508
• Supplier Catalog Number: A28508
• Alternative Catalog Number: ABB-A28508-100UL,ABB-A28508-500UL,ABB-A28508-20UL,ABB-A28508-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Mouse
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: Irf-1

Product Description

Enables DNA-binding transcription factor activity, RNA polymerase II-specific and sequence-specific DNA binding activity. Involved in several processes, including defense response to other organism, regulation of T cell activation, and regulation of gene expression. Acts upstream of or within CD8-positive, alpha-beta T cell differentiation, positive regulation of interleukin-12 production, and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Is expressed in several structures, including bone marrow, early conceptus, eye, genitourinary system, and gut. Human ortholog(s) of this gene implicated in asthma, immunodeficiency 117, lung cancer (multiple), smallpox, and stomach cancer (multiple). Orthologous to human IRF1 (interferon regulatory factor 1).

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 37 kDa
• NCBI: 16362
• UniProt: P15314
• Purity: Affinity purification
• Target: Irf1

Application Notes

• Application Dilute: WB,1:1000 - 1:6000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.For high-ratio antibody dilutions (1:10000),a sequential dilution method is strongly recommended to ensure
• Application Notes: Cross-Reactivity: Mouse. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cell Biology Developmental Biology,Apoptosis,Cardiovascular. Shipping: Ice Bag

Images

Western blot analysis of lysates from RAW 264.7 cells using IRF1 Rabbit pAb (A28508) at 1:3000 dilutionincubated overnight at 4°C. RAW 264.7 cells were treated with mIFN-gamma(100 ng/mL) for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20 s.