SIRT1 Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: SIRT1 Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A28514
• Supplier Catalog Number: A28514
• Alternative Catalog Number: ABB-A28514-500UL,ABB-A28514-1000UL,ABB-A28514-100UL,ABB-A28514-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Mouse
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: Sir2, Sir2a, SIR2L1, Sir2alpha

Product Description

This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 80 kDa/60 kDa
• NCBI: 93759
• UniProt: Q923E4
• Purity: Affinity purification
• Target: SirT1

Application Notes

• Application Dilute: WB,1:1000 - 1:3000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.For high-ratio antibody dilutions (1:10000),a sequential dilution method is strongly recommended to ensure
• Application Notes: Cross-Reactivity: Mouse. ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Mitochondrial metabolism,AMPK Signaling Pathway. Shipping: Ice Bag

Images

Western blot analysis of lysates from C2C12 cells using SIRT1 Rabbit pAb (A28514) at 1:3000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90 s.