Caveolin-1 Mouse mAb, Unconjugated

Article Name:	Caveolin-1 Mouse mAb, Unconjugated
Biozol Catalog Number:	ABB-A28537
Supplier Catalog Number:	A28537
Alternative Catalog Number:	ABB-A28537-20UL, ABB-A28537-100UL
Manufacturer:	ABclonal
Host:	Mouse
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	CGL3, PPH3, BSCL3, LCCNS, VIP21, MSTP085

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.

Molecular Weight:	17 kDa/20 kDa
NCBI:	857
UniProt:	Q03135
Purity:	Affinity purification
Target:	CAV1

Application Dilute:	WB,1:1000 - 1:3000\|IF/ICC,1:100-1:200\|IHC-P,1:1500 - 1:6000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.For high-ratio antibody dilutions (1:10000),a sequential dilution
Application Notes:	Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Cell Adhesion,Endocrine Metabolism,Lipid Metabolism,Cholesterol Metabolism,Insulin Receptor Signaling Pathway,Cardiovascular,Hypoxia,Lipids.

	Western blot analysis of various lysates using Caveolin-1 Mouse mAb (A28537) at 1:1500 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5 s.
	Western blot analysis of lysates from A549 cells using Caveolin-1 Mouse mAb (A28537) at 1:1500 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Caveolin-1 Mouse mAb (A28537) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Caveolin-1 Mouse mAb (A28537) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse pancreas tissue using Caveolin-1 Mouse mAb (A28537) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Caveolin-1 Mouse mAb (A28537) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of HeLa cells using Caveolin-1 Mouse mAb (A28537, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of NIH/3T3 cells using Caveolin-1 Mouse mAb (A28537, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of PC-12 cells using Caveolin-1 Mouse mAb (A28537, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.