ENO2 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A3118
Article Name: ENO2 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A3118
Supplier Catalog Number: A3118
Alternative Catalog Number: ABB-A3118-100UL,ABB-A3118-500UL,ABB-A3118-20UL,ABB-A3118-1000UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: NSE, HEL-S-279, ENO2
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Clonality: Polyclonal
Molecular Weight: 47kDa
NCBI: 2026
UniProt: P09104
Purity: Affinity purification
Sequence: MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERE
Target: ENO2
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Tumor biomarkers,Cell Biology Developmental Biology,Endocrine Metabolism,Carbohydrate metabolism,Neuroscience, Cell Type Marker,Stem Cells,Neuron marker. Shipping: Ice Bag
Western blot analysis of various lysates, using ENO2 Rabbit pAb (A3118) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.