AKT1S1/PRAS40 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: AKT1S1/PRAS40 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A3391
• Supplier Catalog Number: A3391
• Alternative Catalog Number: ABB-A3391-100UL,ABB-A3391-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: Lobe, PRAS40, AKT1S1/PRAS40

Product Description

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 27 kDa
• NCBI: 84335
• UniProt: Q96B36
• Purity: Affinity purification
• Target: AKT1S1

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Autophagy,Cell Cycle,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,T Cell Receptor Signaling Pathway,Neuroscience. Shipping: Ice Bag

Images

Western blot analysis of various lysates using AKT1S1/PRAS40 Rabbit mAb (A3391) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20 s.