NEDD4 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: NEDD4 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A4385
• Supplier Catalog Number: A4385
• Alternative Catalog Number: ABB-A4385-100UL,ABB-A4385-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: RPF1, NEDD4-1, NEDD4

Product Description

This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 149kDa
• NCBI: 4734
• UniProt: P46934
• Purity: Affinity purification
• Target: NEDD4

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Signal Transduction,Cell Biology Developmental Biology,Cell Cycle,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Endocrine Metabolism,Mitochondrial metabolism,Immunology Inflammation,Neuroscience. Shipping: Ice Bag

Images

Western blot analysis of various lysates using NEDD4 Rabbit mAb (A4385) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.