LEF1 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: LEF1 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A4473
• Supplier Catalog Number: A4473
• Alternative Catalog Number: ABB-A4473-20UL,ABB-A4473-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: LEF-1, TCF10, TCF7L3, TCF1ALPHA, LEF1

Product Description

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 44kDa
• NCBI: 51176
• UniProt: Q9UJU2
• Purity: Affinity purification
• Target: LEF1

Application Notes

• Application Dilute: WB,1:250 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cancer,Tumor suppressors,Cell Biology Developmental Biology,Cell Adhesion,Wnt -Catenin Signaling Pathway,Stem Cells. Shipping: Ice Bag

Images

Western blot analysis of various lysates using LEF1 Rabbit mAb (A4473) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.