AFF4 Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: AFF4 Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A4644
• Supplier Catalog Number: A4644
• Alternative Catalog Number: ABB-A4644-100UL,ABB-A4644-20UL,ABB-A4644-1000UL,ABB-A4644-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: MCEF, CHOPS, AF5Q31, AFF4

Product Description

The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5,11)(q31,q31q23).

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 127kDa
• NCBI: 27125
• UniProt: Q9UHB7
• Purity: Affinity purification
• Sequence: MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANW
• Target: AFF4
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cell Biology Developmental Biology,Cell Cycle. Shipping: Ice Bag

Images

Western blot analysis of various lysates using AFF4 Rabbit pAb (A4644) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25µg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.