ALAS1 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: ALAS1 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A4817
• Supplier Catalog Number: A4817
• Alternative Catalog Number: ABB-A4817-20UL,ABB-A4817-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ALAS, MIG4, ALAS3, ALASH, ALAS-H, ALAS1

Product Description

This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme, a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 71kDa
• NCBI: 211
• UniProt: P13196
• Purity: Affinity purification
• Target: ALAS1

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human. ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Cardiovascular,Blood. Shipping: Ice Bag

Images

Western blot analysis of various lysates using ALAS1 Rabbit mAb (A4817) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.