KPNA2 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: KPNA2 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A5012
• Supplier Catalog Number: A5012
• Alternative Catalog Number: ABB-A5012-100UL,ABB-A5012-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: QIP2, RCH1, IPOA1, PTAC58, SRP1alpha, SRP1-alpha, KPNA2

Product Description

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 58kDa
• NCBI: 3838
• UniProt: P52292
• Purity: Affinity purification
• Target: KPNA2

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Immunology Inflammation. Shipping: Ice Bag

Images

Western blot analysis of various lysates using KPNA2 Rabbit mAb (A5012) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.