p47phox/NCF1 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: p47phox/NCF1 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A5143
• Supplier Catalog Number: A5143
• Alternative Catalog Number: ABB-A5143-20UL,ABB-A5143-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CGD1, NCF1A, NOXO2, p47phox, SH3PXD1A, p47phox/NCF1

Product Description

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 45kDa
• NCBI: 653361
• UniProt: P14598
• Purity: Affinity purification
• Target: NCF1

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human. ResearchArea: Cancer,Endocrine Metabolism. Shipping: Ice Bag

Images

Western blot analysis of lysates from Raji cells, using p47phox/NCF1 Rabbit mAb (A5143) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.