WIF1 Rabbit mAb, Unconjugated, Monoclonal

Catalog Number: ABB-A5171
Article Name: WIF1 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number: ABB-A5171
Supplier Catalog Number: A5171
Alternative Catalog Number: ABB-A5171-100UL,ABB-A5171-20UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: WIF-1, WIF1
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Clonality: Monoclonal
Molecular Weight: 42kDa
NCBI: 11197
UniProt: Q9Y5W5
Purity: Affinity purification
Target: WIF1
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cell Biology Developmental Biology,Wnt -Catenin Signaling Pathway,Stem Cells. Shipping: Ice Bag
Western blot analysis of various lysates using WIF1 Rabbit mAb (A5171) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.