ALKBH3 Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: ALKBH3 Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A5808
• Supplier Catalog Number: A5808
• Alternative Catalog Number: ABB-A5808-100UL,ABB-A5808-20UL,ABB-A5808-500UL,ABB-A5808-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ABH3, PCA1, DEPC1, hABH3, DEPC-1, ALKBH3

Product Description

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 33kDa
• NCBI: 221120
• UniProt: Q96Q83
• Purity: Affinity purification
• Sequence: MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRVIDREGVYEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDITYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADW
• Target: ALKBH3
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:2000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse. ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair. Shipping: Ice Bag

Images

Western blot analysis of lysates from Mouse heart, using ALKBH3 Rabbit pAb (A5808) at 1:4000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.