PPARalpha Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: PPARalpha Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A6697
• Supplier Catalog Number: A6697
• Alternative Catalog Number: ABB-A6697-500UL,ABB-A6697-20UL,ABB-A6697-1000UL,ABB-A6697-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PPAR, NR1C1, hPPAR, PPARalpha, PPAR-alpha, PPARalpha

Product Description

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers, this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 52kDa
• NCBI: 5465
• UniProt: Q07869
• Purity: Affinity purification
• Target: PPARA

Application Notes

• Application Dilute: WB,1:2000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Nuclear Receptor Signaling,Nuclear hormone receptors,Cancer,Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Lipid Metabolism,Nucleotide metabolism,Cardiovascular,Hypoxia,Lipids,Fatty Acids. Shipping: Ice Bag

Images

Western blot analysis of various lysates using PPARalpha Rabbit pAb (A6697) at 1:3000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.