PRP19 Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: PRP19 Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A6706
• Supplier Catalog Number: A6706
• Alternative Catalog Number: ABB-A6706-100UL,ABB-A6706-20UL,ABB-A6706-500UL,ABB-A6706-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PSO4, SNEV, PRP19, UBOX4, hPSO4, NMP200

Product Description

Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling, cellular protein metabolic process, and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm, nuclear speck, and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 55kDa
• NCBI: 27339
• UniProt: Q9UMS4
• Purity: Affinity purification
• Sequence: MSLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIRPKPPSATSIPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGLIVPQAVPSSQPSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSAS
• Target: PRPF19
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,Cell Biology Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway. Shipping: Ice Bag

Images

Western blot analysis of lysates from SW620 cells, using PRP19 Rabbit pAb (A6706) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.