NEK2 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: NEK2 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A6811
• Supplier Catalog Number: A6811
• Alternative Catalog Number: ABB-A6811-100UL,ABB-A6811-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: NLK1, RP67, NEK2A, HsPK21, PPP1R111, NEK2

Product Description

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 52kDa
• NCBI: 4751
• UniProt: P51955
• Purity: Affinity purification
• Target: NEK2

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Signal Transduction,Kinase,Cell Biology Developmental Biology,Cell Cycle,Centrosome. Shipping: Ice Bag

Images

Western blot analysis of various lysates using NEK2 Rabbit mAb (A6811) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.