Enables identical protein binding activity. Predicted to be involved in several processes, including cilium assembly, regulation of exocytosis, and smoothened signaling pathway. Predicted to act upstream of or within regulation of L-glutamate import across plasma membrane and regulation of protein localization. Located in several cellular components, including cytoskeleton, photoreceptor connecting cilium, and sperm annulus. Part of septin complex.
Western blot analysis of various lysates, using Septin 2 Rabbit mAb (A7163) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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